Dyskerin Antibody

Catalog Number:

33605

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: O60832
NCBI Gene ID: 1736

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from Internal of human Dyskerin.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total Dyskerin protein.

Applications:

WB IF

Background:

Isoform 1:Required for ribosome biogenesis and telomere maintenance. Probable catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs. Also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.Isoform 3:Promotes cell to cell and cell to substratum adhesion, increases the cell proliferation rate and leads to cytokeratin hyper-expression (when overexpressed in HeLa cells).

Heiss N.S., Nat. Genet. 19:32-38(1998).
Knight S.W., Am. J. Hum. Genet. 65:50-58(1999).
Hassock S., Genomics 55:21-27(1999).

References:

appl_detail:

Western blotting: 1:500~1:3000

Immunofluorescence: 1:100~1:500

Datasheet PDF