MED14 Antibody

Catalog Number:

33592

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: O60244
NCBI Gene ID: 9282

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from internal of human MED14.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total MED14 protein.

Applications:

IHC IF

Background:

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Yoshikawa H., Genomics 49:237-246(1998).
Ryu S., Nature 397:446-450(1999).
Rachez C., Nature 398:824-828(1999).

References:

appl_detail:

Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500

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