XRCC3 Antibody

Catalog Number:

33548

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: O43542
NCBI Gene ID: 7517

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from Internal of human XRCC3.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total XRCC3 protein.

Applications:

WB IHC

Background:

Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Part of the RAD21 paralog protein complex CX3 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, CX3 acts downstream of RAD51 recruitment; the complex binds predominantly to the intersection of the four duplex arms of the Holliday junction (HJ) and to junctions of replication forks. Involved in HJ resolution and thus in processing HR intermediates late in the DNA repair process; the function may be linked to the CX3 complex and seems to involve GEN1 during mitotic cell cycle progression. Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and RAD51C.

Hitoshi Kurumizaka, Nucleic Acids Res., Jul 2003; 31: 4041 - 4050.
Anna Renglin Lindh, Hum. Mol. Genet., Apr 2006; 15: 1217 - 1224.
Zhi-Yuan Xu, J. Pharmacol. Exp. Ther., Aug 2005; 314: 495 - 505.
Nazumi Alice Yamada, J. Biol. Chem., May 2004; 279: 232

References:

appl_detail:

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Datasheet PDF