XRCC2 Antibody

Catalog Number:

33547

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: O43543
NCBI Gene ID: 7516

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from internal of human XRCC2.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total XRCC2 protein.

Applications:

WB IHC

Background:

Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA.

JP Scott, Blood, Oct 1981; 58: 665 - 669.
G Chevalier, Am. J. Pathol., Jun 1998; 152: 1563.
R Williams, J. Biol. Chem., Aug 1993; 268: 18213 - 18217.
Michael R. Ladomery, J. Biol. Chem., Dec 1999; 274: 36520.

References:

appl_detail:

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Datasheet PDF