Sumo1 Antibody

Catalog Number:

33525

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P63165
NCBI Gene ID: 7341

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from N-terminal of human SUMO-1.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total SUMO-1 protein.

Applications:

WB IHC IF

Background:

Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development.

Margarita Vigodner, Am J Physiol Endocrinol Metab, May 2006; 290: E1022 - E1033.
Adrian Minty, J. Biol. Chem., Nov 2000; 275: 36316.
Deborah A. Sampson, J. Biol. Chem., Jun 2001; 276: 21664 - 21669.
Hidehisa Takahashi, J. Biol. Chem., Feb 2005; 280: 56

References:

appl_detail:

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500

Datasheet PDF