PIAS2 Antibody

Catalog Number:

33516

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: O75928
NCBI Gene ID: 9063

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from Internal of human PIAS2.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total PIAS2 protein.

Applications:

WB

Background:

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha. Isoform PIAS2-alpha sumoylates PML at'Lys-65' and 'Lys-160'.

Christina-Maria Hecker, J. Biol. Chem., Jun 2006; 281: 16117 - 16127.
Daria Donati, J. Immunol., SepHeitham Hassoun, 2006; 177: 3035 - 3044.
Heitham Hassoun, Am J Physiol Renal Physiol, Feb 2007; 10.1152.
David J. Sharkey, Mol. Hum. Reprod., Jul 2007;

References:

appl_detail:

Western blotting: 1:500~1:3000

Datasheet PDF