PIAS1 Antibody

Catalog Number:

33515

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: O75925
NCBI Gene ID: 8554

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from Internal of human PIAS1.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total PIAS1 protein.

Applications:

WB IHC

Background:

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. Sumoylates PML (at'Lys-65' and 'Lys-160') and PML-RAR and promotes their ubiquitin-mediated degradation. PIAS1-mediated sumoylation of PML promotes its interaction with CSNK2A1/CK2 which in turn promotes PML phosphorylation and degradation By similarity.

Bin Liu, Mol. Cell. Biol., Feb 2005; 25: 1113 - 1123.
Hansol Lee, Genes & Dev., Apr 2006; 20: 784 - 794.
Tamar Megidish, J. Biol. Chem., Mar 2002; 277: 8255 - 8259.
Keiko Kawai-Kowase, Mol. Cell. Biol., Sep 2005; 25: 8009 - 8023.

References:

appl_detail:

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Datasheet PDF