NEDD8 Antibody

Catalog Number:

33513

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q15843
NCBI Gene ID: 4738

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from Internal of human NEDD8.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total NEDD8 protein.

Applications:

WB IHC

Background:

Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.

Margaret A. Read, Mol. Cell. Biol., Apr 2000; 20: 2326 - 2333.
Kenneth Wu, J. Biol. Chem., Aug 2003; 278: 28882 - 28891.
Katsumi Kito, J. Biol. Chem., Jun 2001; 276: 20603 - 20609.

References:

appl_detail:

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Datasheet PDF