Sirp α1 Antibody

Catalog Number:

33486

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P78324
NCBI Gene ID: 140885

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from human SIRP-alpha1.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total Sirp α1 protein.

Applications:

WB IHC

Background:

Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function By similarity. Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.

Kentaro Ide, PNAS, Mar 2007; 104: 5062 - 5066.
Thomas T. Chen, Cancer Res., Jan 2004; 64: 117 - 127.
Shu Q. Liu, J. Biol. Chem., Nov 2005; 280: 39294 - 39301.
Gurpreet S. Kapoor, Cancer Res., Sep 2004; 64: 6444 - 6452.

References:

appl_detail:

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Datasheet PDF