GAD1/2 Antibody

Catalog Number:

33381

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q4G154
NCBI Gene ID: 2571/2572

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from human GAD1/GAD2.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total GAD1/GAD2 protein.

Applications:

WB IHC

Background:

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form.

JB Travers, Chem Senses, Feb 2007; 32: 129 - 137.
Rita Sulahian, Nucleic Acids Res., Mar 2006; 34: 1351 - 1357.
Andrea Pillai, Development, Jan 2007; 134: 357 - 366.
Dmytro P. Yevtushenko, J. Exp. Bot., Aug 2003; 54: 2001 - 2002.

References:

appl_detail:

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Datasheet PDF