E2F6 Antibody

Catalog Number:

33362

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: O75461
NCBI Gene ID: 1876

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from human E2F6.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total E2F6 protein.

Applications:

WB IHC

Background:

Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase.

Matthew J. Oberley, J. Biol. Chem., Oct 2003; 278: 42466 - 42476.
Claire Attwooll, J. Biol. Chem., Jan 2005; 280: 1199 - 1208.
Paloma H. Giangrande, Genes & Dev., Dec 2004; 18: 2941 - 2951.
J?rg Storre, J. Biol. Chem., Dec 2005; 280: 41380 - 41386.

References:

appl_detail:

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Datasheet PDF