Cytochrome c Antibody

Catalog Number:

33354

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P99999
NCBI Gene ID: 54205

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from human Cytochrome c.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total Cytochrome c protein.

Applications:

WB IHC

Background:

Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.

R. Pullerits, Rheumatology, Jan 2005; 44: 32 - 39.
Bogdan Polevoda, J. Biol. Chem., Jun 2000; 275: 20508
Larry A. Allen, J. Biol. Chem., Jan 1995; 270: 110.
Akemi MATSUSHIMA, J. Biochem. (Tokyo), Jan 1986; 100: 543 - 551.

References:

appl_detail:

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Datasheet PDF