Cullin 2 Antibody

Catalog Number:

33348

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q13617
NCBI Gene ID: 8453

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from human Cullin 2.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total Cullin 2 protein.

Applications:

WB IHC IF

Background:

Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 By similarity. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).

Seiji Shiraishi, J. Biol. Chem., Mar 2007; 282: 9017 - 9028.
Isabelle Groulx, J. Biol. Chem., Mar 2000; 275: 8991.
Dimitris Liakopoulos, PNAS, May 1999; 96: 5510.
Kyungwon Huh, AACR Meeting Abstracts, Apr 2006; 2006: 1326.

References:

appl_detail:

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500

Datasheet PDF