Fos Antibody

Catalog Number:

33336

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P01100
NCBI Gene ID: 2353

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from human Fos.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total Fos protein.

Applications:

WB IHC

Background:

Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.

Qi Mei, Am J Physiol Endocrinol Metab, Oct 2001; 281: E655 - E664.
Jennifer R. Brown, Mol. Cell. Biol., Sep 1998; 18: 5609 - 5619.
T K Kerppola, Mol. Cell. Biol., Jun 1993; 13: 3782 - 3791.
L J Ransone, Genes & Dev., Jun 1989; 3: 770 - 781.

References:

appl_detail:

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Datasheet PDF