eIF2B (Ab-67) Antibody

Catalog Number:

33319

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P20042
NCBI Gene ID: 8894

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from N-terminal of human eIF2B.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total eIF2B protein.

Applications:

WB

Background:

eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

Pathak V.K., Cell 54:633-639(1988).
Deloukas P., Nature 414:865-871(2001).
Olsen J.V., Cell 127:635-648(2006).

References:

appl_detail:

Western blotting: 1:500~1:3000

Datasheet PDF