Cyclin D2 (Ab-280) Antibody

Catalog Number:

33283

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P30279
NCBI Gene ID: 894

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized non-phosphopeptide derived from human Cyclin D2 around the phosphorylation site of threonine 280 (A-S-T(p)-P-T).

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total Cyclin D2 protein.

Applications:

WB

Background:

Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex By similarity.

Xiong Y., Genomics 13:575-584(1992).
Palmero I., Oncogene 8:1049-1054(1993).
Kalnine N., Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.

References:

appl_detail:

Western blotting: 1:500~1:3000

Datasheet PDF