HP1 alpha (Ab-92) Antibody

Catalog Number:

33268

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P45973
NCBI Gene ID: 23468

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized non-phosphopeptide derived from human HP1 alpha around the phosphorylation site of serine 92 (R-K-S(p)-N-F).

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total HP1 alpha protein

Applications:

WB IHC

Background:

Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.

Saunders W.S., J. Cell Sci. 104:573-582(1993).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Ye Q., J. Biol. Chem. 271:14653-14656(1996).

References:

appl_detail:

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Datasheet PDF