TOP2A (Ab-1343) Antibody

Catalog Number:

33258

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P11388
NCBI Gene ID: 7153

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized non-phosphopeptide derived from human TOP2A around the phosphorylation site of threonine 1343 (E-K-T(p)-D-D).

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total TOP2A protein.

Applications:

WB

Background:

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes.

Tsai-Pflugfelder M., Proc. Natl. Acad. Sci. U.S.A. 85:7177-7181(1988).
Wasserman R.A., Cancer Res. 53:3591-3596(1993).
Lang A.J., Gene 221:255-266(1998).

References:

appl_detail:

Western blotting: 1:500~1:3000

Datasheet PDF