PEK/PERK (Ab-981) Antibody

Catalog Number:

33247

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q9NZJ5
NCBI Gene ID: 9451

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized non-phosphopeptide derived from human PEK/PERK around the phosphorylation site of threonine 981 (R-H-T(p)-G-Q).

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total PEK/PERK protein.

Applications:

WB IHC

Background:

Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1) By similarity.

Shi Y., J. Biol. Chem. 274:5723-5730(1999).
Sood R., Biochem. J. 346:281-293(2000).
Delepine M., Nat. Genet. 25-406-409(2000).

References:

appl_detail:

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Datasheet PDF