PAK3 (Ab-154) Antibody

Catalog Number:

33245

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: O75914
NCBI Gene ID: 5063

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized non-phosphopeptide derived from human PAK3 around the phosphorylation site of serine 154 (Y-M-S(p)-F-T).

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total PAK3 protein.

Applications:

WB

Background:

Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development.

Allen K.M., Nat. Genet. 20:25-30(1998).
Jiang C., Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases.
Kitano T., Mol. Biol. Evol. 20:1281-1289(2003).

References:

appl_detail:

Western blotting: 1:500~1:3000

Datasheet PDF