EPHB1/2/3/4 (Ab-600/602/614/596) Antibody

Catalog Number:

33227

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P54762/P29323/P54753/P54760
NCBI Gene ID: 2047/2048/2049/2050

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized non-phosphopeptide derived from human EPHB1/2/3/4 around the phosphorylation site of tyrosine 600/602/614/596 (F-T-Y(p)-E-D).

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total EPHB1/2/3/4 protein.

Applications:

WB

Background:

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Cognate/functional ephrin ligands for this receptor include EFNB1, EFNB2 and EFNB3. During nervous system development, regulates retinal axon guidance redirecting ipsilaterally ventrotemporal retinal ganglion cells axons at the optic chiasm midline. This probably requires repulsive interaction with EFNB2. In the adult nervous system together with EFNB3, regulates chemotaxis, proliferation and polarity of the hippocampus neural progenitors. Beside its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and synapse formation. May also regulate angiogenesis. More generally, may play a role in targeted cell migration and adhesion. Upon activation by EFNB1 and probably other ephrin-B ligands activates the MAPK/ERK and the JNK signaling cascades to regulate cell migration and adhesion respectively.

Tang X.X.,Genomics 29:426-437(1995).
Stein E.,Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases.
Zhang Z.,Protein Sci. 13:2819-2824(2004).

References:

appl_detail:

Western blotting: 1:500~1:3000

Datasheet PDF