B-RAF (Ab-446) Antibody

Catalog Number:

33226

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P15056
NCBI Gene ID: 673

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized non-phosphopeptide derived from human B-RAF around the phosphorylation site of serine 446 (R-D-S(p)-S-D).

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total B-RAF protein.

Applications:

WB IHC

Background:

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.

Stephens R.M., Mol. Cell. Biol. 12:3733-3742(1992).
Albert S., Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
Hillier L.W., Nature 424:157-164(2003)

References:

appl_detail:

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Datasheet PDF