MCM5 Antibody

Catalog Number:

33432

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P33992
NCBI Gene ID: 4174

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from human MCM5 antibody.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total MCM5 protein.

Applications:

WB IHC IF

Background:

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity By similarity. Interacts with MCMBP.

Robert A. Sclafani, Genetics, May 2002; 161: 47.
Marylynn Snyder, PNAS, Oct 2005; 102: 14539 - 14544.
Christopher J. DaFonseca, PNAS, Mar 2001; 98: 3034.
Cathleen M. Lake, Genetics, Jun 2007; 10.1534

References:

appl_detail:

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500

Datasheet PDF