IKK-γ (Ab-85) Antibody

Catalog Number:

33179

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q9Y6K9
NCBI Gene ID: 8517

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized non-phosphopeptide derived from human IKK-γ around the phosphorylation site of serine 85 (Q-A-S(p)-Q-R).

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total IKK-γ protein.

Applications:

WB IF

Background:

Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. However, the specific type of polyubiquitin recognized upon cell stimulation (either 'Lys-63'-linked or linear polyubiquitin) and its functional importance is reported conflictingly. Also considered to be a mediator for TAX activation of NF-kappa-B. Could be implicated in NF-kappa-B-mediated protection from cytokine toxicity. Essential for viral activation of IRF3. Involved in TLR3- and IFIH1-mediated antiviral innate response; this function requires 'Lys-27'-linked polyubiquitination.

Li Y., Proc. Natl. Acad. Sci. U.S.A. 96:1042-1047(1999).
Jin D.-Y., J. Biomed. Sci. 6:115-120(1999).
Rothwarf D.M., Nature 395:297-300(1998).

References:

appl_detail:

Western blotting: 1:500~1:3000

Immunofluorescence: 1:100~1:500

Datasheet PDF