RFA2 (Ab-21) Antibody

Catalog Number:

33162

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P15927
NCBI Gene ID: 6118

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized non-phosphopeptide derived from human RFA2 around the phosphorylation site of threonine 21 (G-Y-T(p)-Q-S).

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total RFA2 protein

Applications:

WB IHC

Background:

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage the RPA complex controls DNA repair and DNA damage checkpoint activation. It is required for the recruitment of the DNA double-strand break repair factors RAD52 and RAD51 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP which is involved in replication fork restart to sites of DNA damage. May also play a role in telomere maintenance.

Erdile L.F., J. Biol. Chem. 265:3177-3182(1990).
Gregory S.G., Nature 441:315-321(2006).
Niu H., J. Biol. Chem. 272:12634-12641(1997).
Cho J.M., Nucleic Acids Res. 28:3478-3485(2000)

References:

appl_detail:

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Datasheet PDF