Bax (Ab-167) Antibody

Catalog Number:

33148

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q07812
NCBI Gene ID: 581

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized non-phosphopeptide derived from human Bax around the phosphorylation site of threonine 167 (F-G-T(p)-P-T).

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total Bax protein

Applications:

WB IF

Background:

Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.

Oltvai Z.N., Cell 74:609-619(1993).
Cuddeback S.M., J. Biol. Chem. 276:20559-20565(2001).
Guo B., Nature 423:456-461(2003)

References:

appl_detail:

Western blotting: 1:500~1:3000

Immunofluorescence: 1:100~1:500

Datasheet PDF