4E-BP1 (Ab-69) Antibody

Catalog Number:

33128

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q13541
NCBI Gene ID: 13685

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized non-phosphopeptide derived from human 4E-BP1 around the phosphorylation site of threonine 70 (A-K-T-P-P).

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total 4E-BP1 protein.

Applications:

WB IHC

Background:

Regulates eIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.

Anne-Claude Gingras, Genes & Dev., Jun 1999; 13: 1422.
Guangming Liu, J. Biol. Chem., Mar 2002; 277: 8810 - 8816.
Ya-Ping Jiang, J. Biol. Chem., Mar 2001; 276: 10943 - 10951.
Anne-Claude Gingras, Genes & Dev., Feb 1998; 12: 502.

References:

appl_detail:

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Datasheet PDF