SOD2 Antibody

Catalog Number:

32265

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot:P04179
NCBI Gene ID:6648

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Recombinant protein of human SOD2.

Purification:

Antibodies were purified by affinity purification using immunogen.

Specificity/Sensitivity:

The antibody detects endogenous level of total SOD2 protein.

Applications:

WB IHC

Background:

The superoxide dismutase family is composed of three metalloenzymes (SOD-1, SOD-2 and SOD-3) that catalyze the oxido-reduction of reactive oxygen species (ROS) such as superoxide anion. The SOD-2 precursor is a 222 amino acid protein that is encoded by nuclear chromatin, synthesized in the cytosol and imported posttranslationally into the mitochondrial matrix. Unlike SOD-1, which is a homodimeric cytosolic Cu-Zn enzyme, SOD-2 is a homotetrameric manganese enzyme (also known as MnSOD) that functions in the mitochondrion. ROS are implicated in a wide range of degenerative processes, including Alzheimer¡¯s disease, Parkinson¡¯s disease and ischemic heart disease. Homozygous mutant mice, which lack SOD-2, exhibit dilated cardiomyopathy, accumulation of lipid in liver and skeletal muscle, metabolic acidosis, oxidative DNA damage and respiratory chain deficiencies in heart and skeletal muscle. Polymorphisms in the SOD-2 gene have also been implicated in nonfamilial, idiopathic, dilated cardiomyopathy in humans.

References:

appl_detail:

Western blotting: 1:500 - 1:2000
Immunohistochemistry: 1:50 - 1:100

Datasheet PDF