PDGFB Antibody

Catalog Number:

32219

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot:P01127
NCBI Gene ID:5155

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Recombinant protein of human PDGFB.

Purification:

Antibodies were purified by affinity purification using immunogen.

Specificity/Sensitivity:

The antibody detects endogenous level of total PDGFB protein.

Applications:

WB,IHC

Background:

PDGF-BB is a cystine-linked homodimer PDGF family member with key roles in development, cell proliferation, cell survival, and angiogenesis (1,2). PDGF-BB is expressed by vascular endothelium, megakaryocytes and Leydig cells (2). PDGF-BB targets pericytes, fibroblasts, monocytes and other cell types (1-3). PDGF induces fibroblast growth and migration (3) and is a chemoattractant for monocytes and granulocytes. Precursor PDGF-BB is cleaved intracellularly to generate a form that contains a carboxy-terminal stretch that serves to retain PDGF-BB in the extracellular matrix. In a second cleavage event, the carboxy-terminal stretch is removed extracellularly to generate mature PDGF-BB (1,2). PDGF-BB binds to PDGFRβ and induces receptor dimerization. Signaling is through the PI3K/Akt, JNK, and PLCγ pathways (1, 2). PDGF-BB may have a role in some cancer types (2).

References:

appl_detail:

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Datasheet PDF