NRAS/HRAS/KRAS Antibody

Catalog Number:

32216

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot:P01111; P01112; P01116
NCBI Gene ID:3845

Form of Antibody:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage/Stability:

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human NRAS/HRAS/KRAS.

Purification:

The antibody was purified by immunogen affinity chromatography.

Specificity/Sensitivity:

The antibody Recognizes endogenous levels of NRAS/HRAS/KRAS protein.

Applications:

WB IHC

Background:

The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) cycle between active (GTP-bound) and inactive (GDP-bound) forms (1). Receptor tyrosine kinases and G protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway (2-4). GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, research studies have shown that in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway (5). The most common oncogenic Ras mutation found in tumors is Gly12 to Asp12 (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein (5,6).

References:

appl_detail:

Western blotting: 1:500 - 1:1000
Immunohistochemistry: 1:50 - 1:100

Datasheet PDF