ACTN1 Antibody

Catalog Number:

32192

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot:P12814
NCBI Gene ID:87

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Recombinant protein of human ACTN1.

Purification:

Antibodies were purified by affinity purification using immunogen.

Specificity/Sensitivity:

The antibody detects endogenous level of total ACTN1 protein.

Applications:

WB,IHC

Background:

α-Actinin belongs to the spectrin family of cytoskeletal proteins. It was first recognized as an actin cross-linking protein, forming an antiparallel homodimer with an actin binding head at the amino terminus of each monomer. More recently, α-actinin has been shown to interact with a large number of proteins involved in signaling to the cytoskeleton including those involved in cellular adhesion, migration, and immune cell targeting (1). The interaction of α-actinin with intercellular adhesion molecule-5 (ICAM-5) helps to promote neurite outgrowth (2). In osteoblasts, interaction of α-actinin with integrins stabilizes focal adhesions and may protect cells from apoptosis (3). Isoforms 1 and 4 of α-actinin, which are non-muscle isoforms, are present in stress fibers, sites of adhesion and intercellular contacts, filopodia, and lamellipodia. The muscle isoforms 2 and 3 localize to the Z-discs of striated muscle and to dense bodies and plaques in smooth muscle (1).

References:

appl_detail:

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Datasheet PDF