SYT1 Antibody

Catalog Number:

32113

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot:P21579
NCBI Gene ID:6857

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Recombinant protein of human SYT1.

Purification:

Antibodies were purified by affinity purification using immunogen.

Specificity/Sensitivity:

The antibody detects endogenous level of total SYT1 protein.

Applications:

WB,IHC,IF

Background:

Synaptotagmin 1 (SYT1) is an integral membrane protein found in synaptic vesicles thought to play a role in vesicle trafficking and exocytosis (1). Individual SYT1 proteins are composed of an amino-terminal transmembrane region, a central linker region and a pair of carboxy-terminal C2 domains responsible for binding Ca2+ (2). The C2 domains appear to be functionally distinct, with the C2A domain responsible for regulating synaptic vesicle fusion in a calcium-dependent manner during exocytosis while the C2B domain allows for interaction between adjacent SYT1 proteins (3). Because synaptotagmin 1 binds calcium and is found in synaptic vesicles, this integral membrane protein is thought act as a calcium sensor in fast synaptic vesicle exocytosis. Evidence suggests possible roles in vesicle-mediated endocytosis and glucose-induced insulin secretion as well (4,5). SYT1 binds several different SNARE proteins during calcium-mediated vesicle endocytosis and an association between SYT1 and the SNARE protein SNAP-25 is thought to be a key element in vesicle-mediated exocytosis (6).

References:

appl_detail:

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Datasheet PDF