P4HB Antibody

Catalog Number:

32085

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot:P07237
NCBI Gene ID:5034

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Recombinant protein of human P4HB.

Purification:

Antibodies were purified by affinity purification using immunogen.

Specificity/Sensitivity:

The antibody detects endogenous level of total P4HB protein.

Applications:

WB,IHC,IF

Background:

During their synthesis, secretory proteins translocate into the endoplasmic reticulum (ER) where they are post-translationally modified and properly folded. To reach their native conformation, many secretory proteins require the formation of intra- or inter-molecular disulfide bonds (1). This process is called oxidative protein folding. Protein disulfide isomerase (PDI) catalyzes the formation and isomerization of these disulfide bonds (2). Studies on mechanisms of oxidative folding suggest that molecular oxygen oxidizes the ER-protein Ero1, which in turn oxidizes PDI through disulfide exchange (3). This event is then followed by PDI-catalyzed disulfide bond formation in folding proteins (3).

References:

appl_detail:

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:20 - 1:100
IP 1:50 - 1:100

Datasheet PDF