AFP Antibody

Catalog Number:

32006

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot:P02771
NCBI Gene ID:174

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

A synthetic peptide of human AFP .

Purification:

Antibodies were purified by affinity purification using immunogen.

Specificity/Sensitivity:

The antibody detects endogenous level of total AFP protein.

Applications:

WB,IF

Background:

Alpha-fetoprotein (AFP) is a 65 kDa glycoprotein found in the serum of the mammalian fetal liver, yolk sac, and GI tract. While AFP expression in adult cells is low, it is aberrantly expressed in adult liver cancer cells (1,2). The tumor suppressor gene p53 and β-catenin are both involved in the regulation of AFP expression. In normal adult cells, p53 binds to the repressor region of the AFP gene, thereby blocking transcription. Mutations in both p53 and β-catenin are associated with aberrant expression of AFP. Elevated serum levels are predictive of hepatocellular carcinoma (3).

References:

appl_detail:

WB 1:500 - 1:2000
IF 1:50 - 1:200

Datasheet PDF