CALR Antibody

Catalog Number:

31077

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot:P30519
Gene ID:3163

Form of Antibody:

Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.

Storage/Stability:

Immunogen:

Fusion protein corresponding to a region derived from 20-218 amino acids of human calreticulin

Purification:

Specificity/Sensitivity:

The antibody detects endogenous level of total CALR protein.

Applications:

ELISA WB IHC

Background:

Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element.

References:

appl_detail:

Predicted MW: 48kd
ELISA: 1:2000-1:10000
Western blotting: 1:1000-1:5000
Immunohistochemistry: 1:50-1:200

Datasheet PDF